BSc (Hons) Biochemistry & MSc in Biological Electron Microscopy, University of Wales, Aberystwyth, UK. PhD, Interface Biology Group, AO Forschungsinstitut, Davos, Switzerland, supervisor Prof. RG Richards. Postdoctoral Fellow, Faculty of Dentistry, UBC, Vancouver, Canada with Prof. D. Brunette. Postdoctoral Associate, New York Structural Biology Center New York, USA with Prof. D. Stokes. Current, Research Associate, Laboratory of Periodontal Biology, Faculty of Dentistry, UBC, Vancouver, Canada with Profs. H. Larjava & L Hakkinen & Technical Director of Electron Microscopy at the Centre of High Throughput Phenogenomics, UBC, Vancouver, Canada.
Understanding the process of musculoskeletal tissue cell biomaterial interactions in vitro and in vivo for promoting wound healing/ tissue regeneration & minimising fibrous encapsulation. Understanding tissue ultrastructure and its role in tissue function.
I have expertise in techniques to specifically study musculoskeletal cell- biomaterial interactions (fibroblast, epithelial and osteoblast) during wound healing at the phenotype, proteome and genotype level in vitro, in particular with biomaterials used in bone fracture healing, dental implants as well biodegradable polymers used in guided tissue regeneration. A rat connective tissue model has also been developed for studying connective tissue- biomaterial interactions as a correlative approach to confirm in vitro data. I have extensive experience (over 20years) in a wide variety of imaging techniques, in particular, immunofluorescence/immunogold labelling, autoradiography, high-resolution 3D electron and focused ion beam microscopy, and live cell imaging.
Available equipment and techniques:
I have developed a reproducible method to replicate surface topography from metal implants onto biodegradable polymers to study the effect of underlying chemistry versus topography on cell behaviour. These substrates are used for biocompatibility testing approaches using cell adhesion quantification in vitro and in vivo testing in a rat connective tissue model. I have access to a imaging facility with capabilities for both cryo and chemical sample preparation, variable pressure SEM, SEM/FIB for ultra-high resolution 3D imaging, uCT, MALDI mass spectrometry, and white light lazer confocal microscopy.